The 67 SSR markers (see Additional File 1) were screened for polymorphism on seven samples, including five varieties of A. hypogaea, one accession of A. ipaënsis and one accession of A. duranensis.
Symbols with different colors indicate different alleles, and each symbol represents one accession.
One accession of O. rufipogon collected from Taiwan also has purple leaf sheath.
Twenty-two genomic SSR markers were tested in 39 asparagus accessions belonging to ten cultivars and one accession of Asparagus setaceus for determination of genetic diversity.
For phylogenetic analyses, matrices of Xu et al. (2012a) were adopted, keeping one accession for each species (Appendix 1) and ITS and trnL-F regions as separated matrices.
Among wild rice accessions for which bacterial artificial chromosome libraries are available [82], at least one accession appears to have introgressed genes from cultivated rice.
However, an extra minor locus was found in one accession of the G. pescadrensis (Tab074), but not in another accession (Tab004).
The qualitative traits were repeatedly observed under light microscopy with five different leaf sample slides per one accession to confirm character stability.
These include the three Asian rice varieties Kasalath (indica), Shuusoushu (indica), and Kha Mac Kho (japonica) from O. sativa, one accession from the African cultivated rice O. glaberrima, and one accession from each of the wild rice species Oryza rufipogon, Oryza barthii, Oryza glumaepatula, Oryza meridionalis, and Oryza longistaminata [41].
The hierarchical cluster analysis of data using phenotypic distances resulted in 25 clusters, from each cluster, ∼10% or a minimum of one accession was selected to form a mini core, which comprised of 35 accessions.
Each scatter presents one accession.
