Sentence examples for once the final time from inspiring English sources

Once the final time point was collected, the shRNAs from each time point were amplified via PCR from gDNA as described (Ketela et al. 2011) and sequenced using an Illumina HiSequation 2000.

Once kneaded the final time, leave the dough to rest for 45 minutes.

He had packed out the house once again, for the final time.

Once more, for the final time, we boarded Rex's Star Speeder and he made his final first flight.

As we visit Julia Martinez for the final time, thanks once again for the wonderful responses to the Julia Martinez scenario on making the shift from peer to boss.

Studies show that those who get professional help investing in their retirement plan achieve as much as an additional 3.32% rate of return.(1) Further, investing in your retirement plan with the end goal in mind will help once you punch that clock for the final time.

The us-against-the-world syndrome is a necessary part of the job of getting a show on, and in all the excitement and heightened intimacy it is all too easy to forget that the friendships will, for the most part, lose their raison d'etre once the curtain has fallen for the final time.

The final time is objectively chosen on a meaningful quantitative criterion: once agreed on the precision level to reach, t f is identified.

Perhaps for the final time.

Redemption beckons, perhaps for the final time.

The final time was the best.