Sentence examples for once stained from inspiring English sources

"Once stained, the tissue can look bright green under polarized light".

The couple are helped along their way by a young war veteran "still wearing part of his uniform" and bowl merrily across fields once stained with Confederate blood.

"This is a historic election, and I recognize the significance it has for African-Americans and for the special pride that must be theirs tonight," Mr. McCain said, adding, "We both realize that we have come a long way from the injustices that once stained our nation's reputation".

And there can be no argument that the whole country has benefited from the most unlikely of sporting marriages: a game imported from a long-ago foe and planted for a little while on turf once stained with the blood of Irish patriots.

Whole-mounted sectioning (4 μm) of blocks allowed an overall appreciation of DPC-1 tumor foci once stained with hematoxylin and eosin (H&E) and expression of PSMA through IHC with 17G1, as described above.

Once stained, follicles were staged according to Spradling (Spradling, 1993).

Once staining had occurred the reaction was terminated in wash buffer and post-fixed in 10% buffered formalin at 4°C.

Once stain had developed, nonstained cell nuclei were counterstained with hematoxylin.

Once staining was optimal the slides were immersed in distilled water and then dehydrated and mounted.

Although heterogeneity does occur, however, once staining is positive in certain cells, these cells will not respond to arginine deprivation and will continue to proliferate.

After 72 hours the cells were fixed by incubation in ice-cold methanol and immunocytochemical analysis performed using a Ventana Discovery™ automated staining platform with a mouse monoclonal antibody targeting VIL2 (Ezrin) (ab4069, Abcam) Once staining was completed sections were washed in warm soapy water, dehydrated in serial alcohol immersions and mounted using DPX mounting medium.