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(I firmly believe that, in this world of ever-diminishing irreplaceable resources, using a line only once represents the sort of wastefulness our society can ill afford).
The proportion of the target region that was successfully sequenced at least once represents the proportion of the target region that we were able to capture i.e. the completeness of the library.
Combined with the fact that exome coverage is usually very deep, we can safely assume that any k -mer that occurs just once represents an error.
The MDNet programme's use of mobile technology to provide health alerts to nearly all physicians in the country at once represents an advanced emergency response capability that rivals systems worldwide.
We therefore argue that from a statistical point of view the highest resolution level of genetic variation where all genetic entities were or can be sampled at least once represents the level that was exhaustively sampled and is thus the appropriate level for projection.
Similar(50)
He also once represented the mobster Bugsy Siegel.
T.W.A. had once represented the future.
College once represented a bubble protected from the outside world.
But the potent energy they once represented is defused.
Candles once represented state-of-the-art illumination.
The Levy family's lawyer once represented Monica's mother.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com