Once reference expression levels and standard deviations are derived in a large group of healthy individuals, deviations could be identified in target individuals using these endogenous genes for normalization without the need for repeated samples from normal, healthy individuals.
Finally, non-normalized short-read data will be extremely useful for downstream applications such as digital gene expression profiling and detection of alternative transcript structure, once reference models are available from the genome.
Klein forged a place in design history by never once referencing the past in his clothes.
Both Clinton and Obama mentioned John Edwards multiple times in their opening statements, and Obama has mentioned Ted Kennedy twice (once referencing his work on health care, another time mentioning their work together on immigration).
The song's lyrics center around a party and having fun, once referencing to leading a double life with, "You see me on the cover of your magazines, things are always different than the way they seem".
Once references were identified for inclusion by our literature search strategy, they were imported into a custom Java-based program that was used to facilitate the management of references and PDF documents.
Once a reference group is formed, these reference groups establish a competitive relationship, either explicitly or implicitly (Festinger 1954; Merton 1957; Akerlof 1982), such as comparing their history, prospects, positive or negative information, or improved conditions (Weick 1979).
[12] Many people had trouble understanding how a proper name could refer to a person (once the reference is initially fixed) regardless of any beliefs we have about that person.
Once a reference database table is generated, it can be used for every alignment to that reference, and there is no need for it to be regenerated with each alignment.
Once the reference technology is identified we calculate the difference between the long-run marginal cost of the new and the reference technology, which yields the economic cost of an individual energy saving measure.
"Zack Morris wants his phone back," I have heard more than once, in reference to the "Saved by the Bell" preppie's bricklike mobile (a late-model Motorola DynaTac, the first cellphone sold commercially, in 1983).
