Intermediate precision was assessed by the assay of sample sets on three different days (inter-day precision).
Separately, one concentration of extracts (10 mg/mL) and reference compounds (1 mg/mL) were injected three times in one day and on three different days.
For intra-day/inter-day comparison of repeatability, the hydrolysate sample was analyzed three times each on day one and additionally on three different days afterwards.
The blood samples were obtained at the same time of day on three different days.
Repeatability and reproducibility were calculated from the chromatograms of the same samples injected at different time points during one day (n = 3) or on three different days, respectively.
The children were tested on three different days, each one week apart.
Interday and intraday precisions were done by preparing and applying three different concentrations of standards (in triplicate) on the same day and on three different days, respectively.
Three challenge tests were performed on three different days, at the same time of day, at least 48 hours apart.
Additionally, effects of clotting time on the serum concentrations of the various peptides were compared for one individual from whom blood was collected on three different days.
The inter-day assays were carried out on three different days with the same concentration levels for spiked plasma samples.
To test the reproducibility of this assay, repeated experiments were conducted on three different days and performed on five different gold chips during the same day.
