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The POTRA domains for conserved BamA proteins with five POTRA domains were predicted based on their alignment to the sequence A1KRL4_NEIMF, which was aligned with, and the POTRA domains defined based on, the BamA Neisseria gonorrhoeae structure (Noinaj et al. 2013); the alignment with structural information was obtained through phyre2.
Contigs were ordered and oriented based on their alignment to the reference genome C. difficile 630 using NUCMER.
The reads from these SAM files were then separated based on their alignment to the forward or reverse strand.
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This allowed to compare sweet orange gene models (see Methods) and two custom genome annotation tracks, representing the 61 GST encoding transcripts we assembled, and 1,611 ESTs putatively encoding GSTs on the basis of their alignment to genomic sequences.
For plant species with complete genome information, conserved and novel miRNAs can be conveniently identified based on their alignments to the genome and known miRNAs in the miRBase and analysis of their pre-miRNA stem-loop structures.
The accuracy of marker positions in the genome is reflected by the order of scaffolds on the chromosomes as scaffolds were placed on chromosomes based on their alignments to markers.
In this species, great use was made of sheep BAC-end sequences (BES), which were assembled in a consensus manner based on their alignments to cattle, human and dog sequence assemblies.
Gene predictions were classified into levels of confidence based on the extent and quality of their alignments to transcripts, proteins, and genome alignments as described in Additional file 1: Table S2.
To determine whether the keratometric and refractive surgical effects of paired intrastromal corneal ring segments (ICRS) depend on their alignment relative to steep and flat topographic meridians.
Potential board members would self-select or be asked to join based on their alignment with the pact.
"Renzi and Berlusconi's ideal candidate would be a weak figure, politically dependent on their alignment," said Wolfango Piccoli, managing director at Teneo Intelligence.
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CEO of Professional Science Editing for Scientists @ prosciediting.com