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To assess the impact of compositional heterogeneity, we performed Bowker's tests for symmetry in the evolutionary process on the whole alignment [ 34, 35].
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If not noted otherwise, identities are reported for the whole alignments i based on truncated alignments Thirty-six (53.7 %) of the reconstructed genes are homologous to previously functionally characterized genes of WSSV (and other organisms in the case of IAP.
The most highly conserved region in the whole alignment is centered on a Cys residue in the motif Cys-Xaa-Gly (bold type indicates residues found in the catalytic triad of sortases).
The program either reads the whole alignment into memory or leaves parts on file, depending on memory resources available on the specific computer.
We also studied the whole alignment results for comparison.
Most GreenPhyl trees are too large and/or too divergent to create reliable nucleotide alignments and perform dN/ dS-based tests on the whole tree alignment.
Three successive runs of ClonalFrame were performed on the whole genome alignment, each with 20,000 iterations and 10,000 of these discarded as burn-in.
Maximum likelihood (ML) phylogeny reconstruction was performed based on the whole proteome alignment with the PhyML program [ 41], using among-site rate variation of four discrete rate categories, and the rtREV amino acid substitution matrix.
We evaluated the sequence conservation of the miRNAs based on the whole genome alignments of human, rhesus, mouse and 14 other vertebrate species by calculating the per-site conservation probability of the human pre-miRNAs.
For the analysis we used the data from Vista tool [ 20] on the whole genome alignments between four plant species.
Primers and probes for the rRT-PCR were designed from the conserved region of the M1 genome segment 5′ end based on the whole-genome alignment of various ARV strains, including six field variants or novel strains obtained in PA poultry.
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