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We developed the type checker based directly on the typing rules that formalise the type system of Circus.
The encodings relying on the typing using the PPP SMARTS definitions are significantly slower with about 7-8 processed molecules per second.
Y-SNP haplogroup assignments were based on the typing results.
Details on the typing of the c.1629C>T polymorphism (rs1044396) of the CHRNA4 gene can be found in [48].
Equimolar inclusion of this primer (150 nM) in the ARMS-DCA assay yielded correct allele calls for the 12 samples under scrutiny and completely suppressed detectable non-specific 7028C product formation, without observable negative effects on the typing of pure and deliberately admixed 7028C/2706A and 7028T/2706G samples (26- and 32-cycle ARMS).
The threshold was adjusted depending on the overall distribution of dots on the typing profile.
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Prices are $20 to $100, depending on the type.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com