Based on the sequenced cDNA sequences from 3'RACE, new primers were designed, which were then used to clone the full-length cDNAs of some BnWRKY genes.
Strain AK83 harbors two copies on the so-called 'chromosome 3' (pSymA), whereas strain SM11 has one copy on pSmeSM11c (pSymA) and strain C017 has one copy on the sequenced cryptic plasmid pHRC017 (Crook et al., 2012).
Our sequence analyses on the sequenced clones have yielded 55 miRNAs, representing 17 unique conserved miRNAs already discovered computationally.
Several microarray platforms have been developed with probe sequences based on the sequenced Arabidopsis genome.
Genotypes were determined by phylogenetic analyses based on the sequenced partial or full genome sequences.
No other genes were identified in the vicinity of the red clover PPO cluster, although retrotransposons and regions of homology with M. truncatula and Lotus japonicus genomic sequence were found on the sequenced BAC 212 G7.
After sequencing, cytosine-to-thymine mismatches (C→T) between the read and the reference sequence indicate unmethylated cytosines on the sequenced '+' strand.
However the sequence identity of these 30 unigenes (84%) was lower than the sequence identity of the 21 unigenes located on the sequenced contigs through hybridisation (90%).
However there is confidence that the sequence belonged to the genus Helicobacter based on the sequenced product.
The occurrences of each family were determined using RepeatMasker (Smit et al. 1996 2010) on the sequenced genomes of An. algerae with the consensus sequences used as library, and the copy numbers were computed using the tool One_code_to_find_them_all (Bailly-Bechet et al. 2014) (supplementary table S2, Supplementary Material online).
This paper outlines a set of standard procedures for describing genes based on DNA, RNA, and protein sequence information that have been annotated and mapped on the sequenced genome assemblies, as well as those determined by biochemical characterization and/or phenotype characterization by way of forward genetics.
