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The measured values on the second assay were doubled to be equivalent to the serum levels.
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The second assay relies on the ability of cholesterol to block the proteolytic cleavage and nuclear localization of SREBP-2 (Brown and Goldstein, 1997).
The second assay, based on ddPCR, uses an emulsion PCR approach to create many thousands of microvolume PCR reactions, with droplets counted at endpoint for presence or absence of the test and reference PCR products.
Specimens reactive on the first assay were retested with a rapid assay, and results were interpreted as HIV-positive only if both tests were reactive.
The second assay can be used on samples prepared from whole blood, that contain both parasite and host DNAs.
The second assay detected Δ7Smn transcripts.
To the best of our knowledge, the CytoBead technique based on digital fluorescence is the first assay technology available so far which provides the unique opportunity to combine autoAb testing employing tissue and purified antigenic targets in one reaction environment [28].
In contrast, the proportion of satisfactorily converted samples in the first assay on frozen samples was 95.0% with LINE-1, 97.4% with MLH1 and 87.2% with MGMT.
This assay is the first assay developed on single-step platform for nucleic acid detection of HBV and HCV with an extra edge over all other assays by providing inbuilt check for quality of sample.
The first assay focused on the capacity of the WS types to invade, from rare, a numerically dominant population of ancestral SM types marked with GFP.
The first assay can be used on samples containing mostly parasite DNA, like those prepared from infected erythrocytes.
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