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We have examined several cases with more than one type of lesion on the same slide.
To ensure direct comparability of staining intensities, one section each from control and SCARKO mice were mounted on the same slide.
This work represents the first step to creating robust protease screening platforms using noncovalent microarray interface that can easily incorporate a range of compounds on the same slide.
In each case, the intensity and distribution of staining in LCIS-associated myoepithelial cells were compared with normal breast tissue on the same slide.
The biopsies were stained with a specific antibody on the same slide.
Technical and biological repeats were printed on the same slide ('print 1'print
On the same slide, a serial section was treated without the primary antibody as a control for secondary antibody specificity.
To determine intra-slide reproducibility, individual sera were tested on six different arrays on the same slide.
Comparative analysis was performed on control and experimental sections on the same slide and stained with the same antibody preparation.
Melanoma and normal skin sections were also immunostained on the same slide as positive and negative controls, respectively.
The presence of multiple cell lines and treatment conditions on the same slide can be exploited as internal controls for both experimental conditions and probes.
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