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Although a direct comparison of freshly prepared and cryopreserved hepatocytes has not been accomplished on the same preparations, several studies have demonstrated efflux in the same order of magnitude in cryopreserved as freshly prepared cells.
The karyotype was analyzed on the same preparations for chromosomal translocations or other rearrangements.
A reciprocal GISH experiment, using labeled genomic DNA of T. occidentale on the same preparations, led to the stronger hybridization of centromeric regions and faint arms of the other 16 chromosomes, including the two NOR-carrying chromosomes, and supporting T. occidentale as the donor of the NOR-bearing genome.
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Intra and inter-test variability were calculated on six replicates of the same preparation run on three different plates and were 30% and 25%, respectively.
Briefly, equal quantities (8 μg) of the same preparation were loaded on two 1D-PAGE gels and submitted to electrophoresis.
Using electrophysiological and biochemical functional assays on the same preparation of prokaryotic ClC proteins (ClC-ec1) that had been used for X-ray crystallography, this study came to the shocking conclusion that ClC-ec1 is a Cl/H exchange pump, rather than the Cl ion channel initially assumed.
For this purpose, the mean amplitude of the duodenal contractions preceding each distension or agonist superfusion was taken as 100% and no more than 5 distensions or superfusions were performed on the same preparation.
This approach produces an ovariogram in which the contractions at several sites on the same preparation can be recorded and compared.
ML content of VAE was obtained from the respective publication, or, if not available, it was either left out, or calculated from other contemporary publications on the same preparation, or calculated on the basis of information provided by the corresponding manufacturer.
So no fanfare this time around, I just have to get on and race, do the same preparations as before and get the job done.
(The intimacy of the dining room has another advantage: on subsequent visits, they'll make sure you aren't served the same preparations from the ever-changing menu).
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com