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The efficient enzymatic hydrolysis is dependent on the set of enzymes on the cellulosome, with the most effective enzyme composition being dependent on the respective substrate (Blouzard et al. 2010; Chundawat et al. 2011).
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E2s are capable of mediating linkage specificity, either on their own, by the use of a cofactor or with the help of the respective substrate 6, 7.
In the coating process, the absorbances of QDs on the surface of the respective substrates were monitored to maintain similar intensities since absorbance corresponds to PL intensity.
The Chi-square was 25.2 and 28.3 for the respective substrates.
Fig. 9 Typical fluorescent microscopy images of HUVECs cultured for 24 and 72 h on the respective substrates.
Furthermore, note that the detailed imaging of AFM proved less aggregation in Figure 2. When similar amounts of QDs were deposited on the respective substrates, the intensity difference between them was very small, which may strongly support the vital role of the plasmonic rough surface in enhancing fluorescence emission Figure 3D.
The profiles of secreted hydrolytic activities demonstrate the adaptation of the fungi to growth on the respective substrates.
However, the cell attachment study did not provide any biological information regarding the long-term survivability of the Vero cell line on the respective substrates.
To corroborate that, atomic force microscopy was performed on the respective substrates which indeed showed dewetting of casein and collagen over time (data not shown here).
Confocal laser scanning microscopy and SEM results show martensite coarsening in the treated surfaces regarding the respective substrate bulks, which is related to the surface C-alloying, thus being dependent on the treatment time and temperature.
Specific binding was visualized using alkaline phosphatase avidin together with the respective substrate (Life Technologies).
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