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A pH measurement was made on the normalized volume.
SSAA applies the same spot outline to corresponding spots for all gels before the application of ANOVA (p < 0.05) for statistically significant changes in their protein expression based on the normalized volume of each spot.
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For the statistical analysis, we used the log2 of the normalized volumes.
The normalized volume of spots on three replicate 2-D gels was averaged and the standard deviation was calculated.
The normalized volume for each spot on each array was calculated by first removing the background signal estimated by the first quartile of the nonspots, and then log-transformation of the spot volumes was used to generate normally distributed data.
Finally, the normalized volume for each protein spot was compared for all 12 maps.
To address this limitation, we use the normalized volume as suggested by (Klotz et al. 2007).
The normalized volumes were resampled to a voxel size of 3 mm × 3 mm × 3 mm.
The maximum normalized volume of lesions at the metal fibers was 0.655.
The maximum normalized volume of PHBV/PAM scaffold was much smaller than that of PHBV scaffold.
Comparative analyses were carried out with the mean normalized volume between each NFPA subtype and controls.
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