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In Arabidopsis, the T-DNA integration occurs between the recipient genome and microhomologous sequences of the T-DNA composed of 5-bp and 2-bp sequences on the left border and right border, respectively (Brunaud et al. 2002).
In A. aculeatus, microhomology was observed on the left border of the T-DNA; however, the other bias has not been identified thus far, although we are in the process of analyzing more transformants.
While the first cycle of TAIL-PCR, conducted on the left border, generated non-specific amplification products, the last cycle yielded specific products that were only detectable in genomic DNA from transformed mycelia (Figure 4a).
The brightness analysis shows that paxillin proteins on the left border (the disassembling part) are brighter (more clustered) than at other points along the orbit.
Consequently, the duplicated assays of this enzyme appear relatively isolated from its rabbit counterpart on the left border of the plot.
eds5H-1 plants were isolated by using the forward primer JL-202, which is present on the left border of T-DNA region (JL-202) and the reverse primer GSP1.
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They are two points, on the right and the left border of the junction of the base of the skull and the neck.
The shaded area in Fig. 6 displays these differences on the black line at the left border of the shaded area (April 2008) and right border of the shaded area (October 2008).
Segregation of homozygous plants carrying the T-DNA insertion was confirmed on genomic level by PCR, using the left border primer TL1 and the CAT8-specific reverse primer Crv: CCTATGATGTAGCTCATCAGCC. Lack of the transcript in cat8-1 was verified by RT-PCR using Crv and Cfw: CTCggtaccATGATCCCTGCTTCAATGGAG primers.
The Cauliflower mosaic virus (CaMV) 35S promoter (35S), the ribozyme (RZ) sequence, and the nopaline synthase terminator (nos) were constructed and inserted between the left border (LB) and right border (RB) on pBD003.
Touch screen: Swipe into the screen from the left border.
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