Two hydrophobic patches are noted on either side of the conserved hinge segment.
We evaluated the evolutionary conservation of each pathway map, based on the conserved orthologous genes derived as above.
A 1339 bp DNA fragment of the putative oriC region from the rifamycin SV producer Amycolatopsis mediterranei U32 was cloned by PCR amplification employing primers designed based on the conserved flanking genes of dnaA and dnaN.
Using a primer, containing a 3'-terminus based on the conserved RNA segment termini of Uukuniemi virus, clones containing N (EU274386) and NS-s gene (EU274385) sequences of the S RNA segment of Precarious Point virus [3] were obtained.
To clone the S. exigua EcR gene, degenerate primers based on the conserved amino acids sequences of "PRQQEE" (forward primer, SeEcR-F) and "QPSXE" (reverse primer, SeEcR-R) were used to obtain a common fragment of EcR (Table 2).
We therefore developed a sensitive and specific nested PCR assay based on the conserved gltA gene.
We therefore recommend to impose a certain threshold F thres on the conserved family count.
Phylogenetic relationships based on the conserved sequences within Papilionoideae legumes imply that Vigna (cowpea) is closely related to soybean [ 38].
Depending on the conserved amino acids residues, HCRs can be divided in two classes: Class I and Class II.
The evidence for homologous recombination and natural selection pressure whether operate on the conserved coding genes was estimated at the present study.
