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All following steps were conducted on the assembly based on the combined library only.
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Compared to the assembly based on corresponding zinc porphyrin combined with isonicotinic acid by metal-ligand axial coordination, CP-isonicotinic acid assembly exhibits a significantly enhanced photoelectronic behavior.
The 15 bp SAGE tags were mapped to the assembly based on exact sequence matches.
13,523,039 reads were remapped to the assembly based on the following criteria: ≥ 94% sequence identity; ≥ 200 bp non-repeat-masked bp and at least 200 bp of PhredQ ≥ 30 bp.
A comparison of the assemblies based on percent similarity among the final number of unigenes is shown on Figure 3.
We assessed the quality of the assemblies based on the total number of transcripts, open reading frames predicted using Transdecoder and highest mapping percentage of the reads using GSNAP.
Finally, we make a quantitative analysis of the proposed assembly planner in comparison with the assembly based on human's movements only.
The genome sequences of Pseudomonas stutzeri TAIHU and Mesorhizobium sp. TAIHU, can be retrieved from the assembly based on the scaffold list belonging to each species in Additional file 1: Table S7.
As shown in Figures 1 and 2, the assembly based on CP000437 contains large pile-ups of mis-oriented reads (shown in red) flanking each inversion.
Redundant BAC clones were removed from the assembly based on the necessity and the relative contribution of each overlapping BACs on the contig.
The sequences of the 24 individual BACs were extracted from the assembly based on Blastn search against available BAC end sequences (BES).
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