We then evaluated each method using features on the array that assay known sites of differential methylation or genotype.
Results presented in Figure 5 and Table 4 confirmed the prediction based on priming probabilities that Assay 1 has greater discriminatory power than Assay 2. Better discrimination is useful for genotyping assays or molecular diagnostics, whereas less sensitivity to SNPs is useful to buffer the effects of genotypic variation in gene expression assays.
Based on priming probabilities, we predicted that Assay 1 had the greater potential for discriminating between targets and closely interfering molecules.
Taken together, these and other results [12], [29] argue that LKB1 suppresses invasion and metastasis and suggest that assays based on LKB1 may prove useful for prognostication in a variety of cancers.
Our results suggested that assays relying on immunoprecipitation of chromatin will be biased by intrinsic differences between regions packaged into different chromatin structures - biases which have been largely ignored to date.
Several studies suggest that assays based on PCR could serve as a useful tool in diagnosing IMI pathogens [ 15- 17].
These data suggest that assays based on antibody responses to the CPs of Mtb may be of value for the serodiagnosis of TB.
However, it has been recommended that assays based on hydrogen atom transfer and electron transfer reaction together should be used to provide a better view of antioxidant activity than a single method [ 17].
The reason for using two parallel ELISAs in the investigations was the suggestions from the literature that assays based on antigen from C. burnetii isolated from ruminants rather than ticks may be more sensitive [ 10, 12].
The reactions include labeled modifiers (e.g., ubiquitin, SUMO1, or NEDD8), ATP regenerating system, and other required components (depending on the assay) that support the conjugation of the modifier.
