Sentence examples for on target efficiency from inspiring English sources

On target efficiency for the matched sample set (n = 6 samples repeated across each platform) and the unmatched data set (n = 6 different samples per enrichment platform) is provided in Tables 3 and 4. We note that our criteria for what counts as an on-target base is strict, in the sense that the sequence immediately flanking the targeted regions was excluded.

Again, raindance performance is notably lower in the ummatched compared to the matched set; this is largely attributable to one outlier sample that exhibited a lower on-target efficiency.

Cas9 activity was measured through indel detection and relative Cas9 cutting efficiencies were calculated by normalising the off-target cutting efficiency to on-target efficiency.

Agilent SureSelect exhibited superior on-target efficiency and correlation of read depths across samples.

In particular, we observed higher on-target efficiency with Agilent, which ultimately resulted in increased coverage depth performance.

Both Raindance and Nimblegen SeqCap exhibited tighter distributions of read depth around the mean, but both suffered from lower on-target efficiency in our experiments.

As further discussed below, Raindance performance on the unmatched sample set was markedly lower than observed for the matched sample set, primarily due to an outlier sample with low (38%) on-target efficiency.

As indicated in Figure 3, both Raindance and Nimblegen exhibited tighter sequence coverage depth distributions around the mean as compared to Agilent, but the benefits of these tighter distributions were outweighed by lower on-target efficiency that was observed for these platforms in our experiments.

On the effect of folate content on targeting efficiency studied on KB cells, it was observed that the maximum cellular uptake peaked at 65% folate content (Zhao and Yung 2008).

They also suggested that lengthening the 5′ end of gRNA reduces on-target editing efficiency, as 5′ nucleotides might not be necessary for full gRNA activity.

Limited by these factors, current literature offers preliminary insights into the general applicability and on-target methylation efficiency of dCas9-fused methylation effectors yet lacks a general interpretation of global off-target activity.