Exact(3)
He showed that a very good agreement with the observed energy distribution could be obtained if new comets were disrupted about 10 percent of the time on the first perihelion passage from the Oort cloud and about 4 percent of the time on subsequent passages.
VISA and hVISA, strains of S. aureus that contain subpopulations of daughter cells displaying intermediate sensitivity to vancomycin but for which the MICs for vancomycin fall within the susceptible range, can be difficult to detect in the microbiology laboratory because the phenotypes are unstable and can be lost on subsequent passages (12 ); this situation was demonstrated in our case.
We show that the transmission of atypical BSE-H isolates in transgenic mice expressing homologous bovine prion protein (PrP) led to emergence of a clearly distinct prion with strain features similar to those of the BSE-C agent and that such similarities were maintained on subsequent passages.
Similar(57)
Such a signature, termed PrPsCJD because of its similarity with that in sCJD-challenged mice was conserved on subsequent passage (35/35 analyzed; Figure 1A, B, D).
The CPE, which was reproduced on subsequent passage on fresh cell line monolayers, was characterized initially by cell vacuolization and rounding and then detachment from the substrate involving the whole cell monolayer.
However, the failure to maintain the resistant virus on subsequent passage suggests that the pathogenicity of 14-P4 may have been only partially attenuated relative to the sensitive virus and indeed the pathogenicity of the clonal virus isolate, 14-P4E, waseverelyly attenuated.
When selecting for adherence, the adherent bacteria from the previous passage on A549 cells were used to inoculate new cell layers of A549 for subsequent passages.
Cells from positive wells were rescreened on three subsequent passages by IFA, and the positives were frozen in liquid nitrogen.
The highest titer sample from each lineage was diluted and used for subsequent passage (Fig. 1).
Such a switch, that we term "domestication" [ 13] results in the formation of strains that are stable during subsequent passages on agar media and that form smooth colonies similar to those formed by standard S. cerevisiae laboratory strains [ 13].
Subsequent passages were maintained on uncoated dishes in astrocyte medium.
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