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Furthermore, interestingly, we correctly identify the stage of symbiosis for 92%–100% of the proteins contained in modules depending on stringency (see methods and Fig. 3c).
In dorsolateral prefrontal cortex (DLPFC) tissues from 14 individuals, we found evidence for frequent regulatory variants affecting RNA expression in tens to hundreds of genes, depending on stringency for assigning AEI.
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Regardless of the underlying technology, enrichment strategies attempt to strike a balance between on-target stringency and coverage maximization.
Membership computation relies on a fuzzification parameter (r, ranging between one and infinity) acting on the stringency of clustering.
In general, the number of spikes to be used in different applications may vary depending on the stringency of the criteria used, on the desired variability threshold and on the robustness of the platform and it may have to be recalibrated when using other sources of RNA.
Kerner et al. classified the GroEL substrates into Class I, II or III based on the interaction strength and on the stringency of their requirement for GroEL.
Outcomes of experiments depend not only on experimental design but also on the stringency of conditions of the experiments: for example, too stringent conditions can lead to false negative interactions, as opposed to false positive results obtained from experiments that were not stringent enough.
In our case, depending on the imposed stringency on contig coverage, our initial number of potentially useful, perfect microsatellite markers ranged from 139,098 to 85,567 (at least 10X contig coverage), and finally to 1,135 (100X coverage).
Also noteworthy was the discovery of many errors in the on-line bioinformatics databases pertaining to GLTP genes, perpetuated by predictive modeling analyses that relied on low stringency survey data (e.g. exon deletion in the chimp GLTP gene) rather than on carefully executed experiments.
The number of cycles depends mainly on the affinity of the aptamer-target interactions as well as on the stringency imposed to each round of selection.
It has long prided itself on the stringency of its community service requirement for students, for example.
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