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It may depend on stable interactions of tTG with adrenergic receptors [29] or integrins [20].
Notably, this peptide pull-down workflow has been applied to numerous histone PTMs and has provided a vast body of knowledge on stable interactions between nuclear proteins and specific histone marks.
Perhaps because of this, many BiFC studies have focused on stable interactions, for example, between transcription factor dimers (Hu et al., 2002), Gβγ subunits (Mervine et al., 2006) or 7TMR dimers (Briddon et al., 2008; Gandia et al., 2008; Vidi et al., 2008).
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By contrast, facile losses of Sm-E, U1-C and Sm-B/B' are common to all dissociation products and lead to disruption of the ring on opposite sides, revealing stable interactions of Sm-G with Sm-D3 and U1 snRNA.
An investigation of the three-dimensional structures of about 100 yeast proteins indicated that buried residues – which are located on a stable interaction surface between protein units – are under stronger evolutionary constraints than solvent exposed sites [24], even after excluding the effect of expression level.
Cohesin rings lock around the sister DNAs at the nascent replication fork in a manner that depends on the stable interaction of the N terminus of the kleisin subunit with the SMC3 ATPase domain.
If the number of sites on an APC that can form stable interactions with CD8+ T cells are limiting, perhaps CD8+ T cells compete for progression to phase two.
IUPred assesses the tendency of a protein to contain disordered regions based on whether the amino acid sequence allows stable interactions (Dosztanyi et al., 2005).
For each allele, either the most posterior Hoxd13 gene forms stable interactions within the TAD on the centromeric side or, alternatively, the Hoxd11 to Hoxd1 genes interact with the TAD on the telomeric side.
This synapse also shares similarities with the sequential development of cell cell interactions required for efficient leukocyte endothelial cell interaction before extravasation; here, weak interactions lead, via signalling events, to more stable interactions utilising β2-integrins that arrest leukocytes on vascular walls through interaction with adhesion molecules including IgSF members.
These findings suggest that IVV technology can identify both stable and less stable interactions involving disordered regions.
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