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It is therefore possible that detection of H3K27me3 on specific loci may have a function other than transcriptional repression.
Distinct DNA methylation patterns, which can vary between different tissues and developmental stages, exist on specific loci.
Targeted capture of genomic regions reduces sequencing cost while generating higher coverage by allowing biomedical researchers to focus on specific loci of interest, such as exons.
With such genomic features, evidence is gathered at many loci, which make the findings not heavily dependent on specific loci in individual samples.
The approach we present here for modeling the error distribution is based on the observation that sequencing artifacts are recurrent on specific loci.
In this way, these binding sites alleviate selective pressure on specific loci, namely classical regulatory binding sites, and increase the ability to evolve [ 53].
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Substantial numbers of Xmv, Pmv or Mpmv elements were detected in the mice, whereas previous reports have suggested that these elements should be rare among European mice based on Southern blot and restriction digestion experiments [ 9], or based on analysis of specific loci known to carry Xmv, Pmv or Mpmv [ 4].
The nucleus image (a) shows live cell CRISPR labelling of specific loci on human chromosomes 1 and 13 and is provided by H. Ma and T. Pederson.
Unlike H3K9me3 and H3K27me3, which have been associated with repression of specific loci on a genome wide scale, H4K20me3 showed no obvious association with active or repressed promoters [50].
This contrasted with the behaviour of specific loci on labelled chromosomes, which showed more progressive reorganisation, and revealed that "looping out" of chromatin from chromosome territories is a dynamic state.
Next, quantitative real-time PCR (q-PCR) also showed the high enrichment (≥9-fold) on the specific loci after KLF4 ChIP.
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