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Estimations of T.e.CBH1 protein concentration based on total protein and the concentration of active T.e.CBH1 based on specific activity on MULac were fairly consistent, as they were for the Ac.t.CBH1, which was produced at a low level but was evidently capable of cellulose conversion.
Fig. 5 Effect of pH on specific activity and stability of purified GluDH-BA.
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Effect of pH on specific activity of purified GluDH-BA was determined by measuring the activity at 37 °C using CPB buffer with a pH range from 5 to 10.
Cofactor-binding loop 2 variants had detrimental effects on specific activity at elevated temperatures, whereas the H192P mutation in cofactor-binding loop 1 resulted in a two-fold improved stability to inactivation at elevated temperatures, and increased the critical onset temperature for aggregation.
Mutant F264A has a quite moderate effect on specific activity.
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Founded on one specific activity of organising a group gift, the community can have a conversation and exchange comments around the process, before deciding on the gift.
The program implementation component usually does not appear in the form of systematic publications, except for annual reports on specific activities (e.g. the number of speed cameras activated; the number of roundabouts constructed, etc).
Firms can focus on specific activities while in- or out-sourcing others.
Twice every month, caregivers receive calls from Galli Galli Sim Sim with tips on specific activities which can be carried out with the children.
She also plans to obtain more detailed information on specific activities taking place at well sites during the first trimester.
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