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We also thank Eric Tang for assistance on sequencing two BACs using Sanger sequencers.
But it all comes to life when we get to the decision to move from worms to humans and to gamble on sequencing the lot.
"All the current logic is based on sequencing," he said, with humanitarian agencies moving in first to deal with an emergency before development workers take over.
The consortium has spent only $300 million on sequencing the human genome since January 1999, when its all-out production phase began.
"They are making rapid progress on sequencing the mouse.
So we developed a program internally to work on sequencing.
Based on sequencing data, all 19 colonies contained the correct (in frame) VHH fragments.
SUP-MLPA products were analyzed on sequencing gel electrophoresis with extraordinary resolution.
Therefore, identifying a novel influenza is entirely dependent on sequencing its whole genome.
And the momentum is pushing even more proactive solutions based on sequencing individual genomes.
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For example, global nuclear run on-sequencing (GRO-seq) and precision nuclear run on-sequencing (PRO-seq) with modified nucleotides (Core et al., 2008; Kwak et al., 2013) provide a way to study Pol II profiles associated with nascent transcription.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com