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Put the fine and coarse cornmeal on separate plates.
Three replicates of 100 cells each were prepared on separate plates that were incubated at 28 °C for 2 days.
In the "plug assay", we re-streak individual soil colonies on separate plates.
These two assays were carried out as separate reactions on separate plates maintaining the sample positions between the two plates.
Quantitative PCR (Q-PCR) analysis was done in triplicate for each region, on separate plates for genomic and input samples.
Serum and plasma samples were run on separate plates because they require slightly different buffers as indicated above.
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These libraries were sequenced on four separate plates, one library per plate, by 454 GS FLX technology to produce 366 Mbp of sequence.
For reprogramming in different oxygen tensions, the electroporations were split on two separate plates: one cultured in 4% O2 (Biospherix ExVivo chamber) whereas the other in normal room air of around 20% O2.
Analyses were conducted on 20 wells per plate on three separate plates for an n = 3.
We calculated intra and inter-assay coefficients of variance using 3 serum samples run in triplicate on three separate plates.
For the iELISA and the bELISA, the intra-assay repeatability was calculated for 48 replicates on 3 separate plates, then repeated over a 3-day period for inter-assay repeatability assessment.
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