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Approximately 300 μl of cell suspension/growth media was added directly to the top of the cover slips, and cells were plated at varying concentrations (4 × 10 5 × 10 cells per ml) on separate cover slips for each assay to control for variability in antibody affinity between different experiments.
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The March 20 issue of the magazine featured Kobe Bryant, Anna Kournikova, Regis Philbin and Ms. Spears on separate covers under the headline "The New Stars of Money".
Time really, really, really wants to know if animals can think: so much that it's done three separate cover stories on the issue.
Sometimes the grand vizier would append a separate cover page on top of a proposal coming from a lower-level functionary like the Treasurer (Defterdar) or the Minister of Defence (Serasker), introducing it as, for example, "this is the proposal of the Defterdar".
Submit a separate cover letter for each position applied.
Cons: They require a separate cover.
They are inexpensive.. Cons: Require a separate cover.
This week, Another Man magazine unveiled three separate covers for their upcoming issue, scheduled to drop on 29 September.
Alternatively, buy separate covers.
After 5 min incubation in closed microtiter plates maintained at 37°C, about 20 μl of test mixtures were placed on separate microscope slides and covered with 7×22-mm cover slips and the parasites were observed every 5 min for death/motility for a total duration of one h using X400 objective.
Individual single colonies of C. cinerea O7 vegetative mycelium were cultivated on separate 30-ml YMG agar plates covered with sterile cellophane discs at 37° in the dark for 96 hr.
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