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We focused on select cases in which molecular dynamics simulations were used to complement experimental studies aiming to understand the structure and properties of new compounds at the atomistic level.
Immunoperoxidase staining for collagen type III (Cosmo Bio USA, Carlsbad, CA, USA) was performed on select cases.
A descriptive case series of the infants infected with HPeV3 during this outbreak, containing further clinical details on select cases, is reported elsewhere (6 ).
Brain single photon emission computed tomography (SPECT) using Tc-99 m-ECD (Neurolite) as the radiotracer was performed on select cases, as was brain PET using fluorodeoxyglucose (FDG) as the radiotracer, and the patterns of hypoperfusion (for SPECT) and hypometabolism (for PET) were noted.
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Investigations on selected cases at the molecular level are discussed further.
On selected cases a psychological consultation can be of great help to better understand the patient and can protect the surgeon from unpleasant litigation.
The western blot data on selected cases for p-AKT, p-4E-BP1 and p-p70S6K were in complete concordance with the IHC results.
Immunohistochemical staining of CD30 was performed on selected cases using a CD30 antibody (Invitrogen, Carlsbad, CA, USA) incubated at a 1 50 dilution.
In addition, double-labeling immunofluorescence was carried out on selected cases for combinations of: platelet derived growth factor receptor (PDGFR α/β, PDGFRα/glial fibrillary acidic protein (GFAP), PDGFRα/CD45, and PDGFRα/HLADR.
Following analysis of the AT8 staining, Gallyas silver staining from temporal and occipital sections was carried out on selected cases from each Braak stage to confirm reliability of AT8 immunostaining and staging (data not shown).
They were instructed on how to select cases, how to define and code the variables and what to do in case of haziness.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com