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Analysis of variance showed that all parameters had significant effects on SDS removal.
Effect of pH value on SDS adsorption and foam properties were studied.
The antibody purities were >95% based on SDS gel electrophoresis analysis.
Protein band at approximately 25 kDa on SDS gel was excised and cut into small chips.
and analyzed on SDS containing polyacrylamide gels.
For Western blotting, samples were loaded on SDS polyacrylamide gel.
Similar(49)
The bound proteins were eluted with SDS sample buffer, fractionated on SDS PAGE visualized by fluorography.
The purified enzyme showed prominent single band on SDS-PAGE.
Purified β-mannanase from M. cinnamomea had molecular mass of ∼60 kDa on SDS-PAGE.
However, the IgG purity remained almost constant at around 95% based on SDS-PAGE analysis.
Cross-linking can be detected readily by a mobility shift on SDS PAGE.
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