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The fraction of autotrophs on the respective media was tabulated to determine recombination frequencies.
The bacteria were grown until an OD600nm of 0.6 was reached and then plated in triplicates on their respective media to confirm the number of bacteria per mL.
Spheroids of SNB19, U251, 5310 and 4910 cells were prepared by seeding a suspension of 5×104 cells in their respective media on ultra low attachment 96-well plates and cultured until spheroid aggregates formed.
For media-shift experiments, cultures were grown in SCGlu and SCGly liquid media to logarithmic (6 h) and stationary (24 h) phase, respectively, and 500 cells were plated on respective solid media plates as well as on SCGlu plates in any case.
The bacterial isolates were sub-cultured on agar slants of their respective media at regular intervals to maintain viability as well as metabolic activities.
The prostate cancer cell lines were cultured on 150 mm plates in their respective media.
Colonies emerging from the transformation plates were subcultivated on respective selective media and subsequently purified by single spore isolation on plates containing additionally 0.1% Triton X-100 for restriction of colony growth.
For S. cerevisiae, auxotrophic derivatives of prototrophic BY4741 (Mülleder et al., 2012) were cultured alone or mixed in combination with other auxotrophs, and 1.1e05 cells of individual or mixed auxotrophs were spotted on respective selective media.
The sentiment of these articles varied significantly, based on variables such as publication date and their respective media outlet.
