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hBMSCs were isolated and cultured based on protocols from previous studies [ 11, 12].
It is based on protocols from mammalian subcellular preparations (Huttner et al., 1983; Ahmed et al., 2013).
A prevalence study based on protocols from maintenance claw trimmings demonstrated that 72% of Swedish dairy cows had at least one claw lesion [ 3].
To assess the biological activity, of the compounds shortlisted by the in vitro screen on HIV-1 replication, we employed a viral replication assay based on protocols from Hu et al. (Table S2, Supporting Information).
Specimens for in situ hybridization were anaesthetized in tricaine methanesulfonate (MS222, Argent) and fixed overnight in 4% paraformaldehyde (PFA) in 0.1% phosphate-buffered saline (PBS) at 4°C. Whole-mount in situ hybridization experiments were based on protocols from [ 12] and modified as follows: embryos were transferred to methanol for dehydration and stored at -20°C.
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Any conclusions based on the quantitative analyses of protocols from a single patient are clearly provisional.
Chemotherapy is, therefore, chosen for an individual patient based on protocols developed from observations from prior clinical studies on other patients.
Summative mass closure of the biomass was obtained following the protocol established by Rocha et al. [ 32], developed specifically for sugarcane bagasse, and based on standard protocols from the National Renewable Energy Laboratory [ 49, 50] and also on a validated methodology from [ 51, 52].
Overall, there is only limited data on chemotherapeutic protocols from which no firm treatment recommendation can be drawn.
The hemolysis and protection assays were performed based on modified protocols from Blocker et. al. and Goure et al. [27], [46].
In Family 1, we isolated mRNA using the RNeasy kit (Qiagen, Hilden, Germany) from lymphoblastoid cell lines that have been established based on standard protocols from patients MR026-01 and MR026-04.
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