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ICI significantly reduced pLUM with no significant effect on pLB.
Kusumoto (1978) suggested that the negative effect of NAA on PLB proliferation in the case of cymbidiums was overcome by BA.
Recent studies on PLB knockout mice have underlined the importance of PLB as a key regulator of cardiac contraction and relaxation [ 5].
As expected triiodothyronine (positive control) increased SERCA2a mRNA levels by approximately 75% as assessed by quantitative RT-PCR and had no effect on PLB expression.
Repeated triggering of single PeD12 spikes on a faster time base revealed the presence of short-latency 1 1 excitatory postsynaptic potentials (EPSPs) on PlB, also consistent with a monosynaptic connection.
Irrespective of such subtle differences, however, the effect of the RNAi vectors in vivo on PLB ablation, haemodynamics, and morphology confirmed that the doses chosen for both systems are within the therapeutic range.
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To determine whether oligomers formed from activated toxin or protoxin are able to induce pore formation, we analysed the pore-formation activity of the membrane-associated proteins using voltage clamp on PLBs as described in the Experimental section.
Fleire et al. can only examine and quantify the efficiency of the internalization of antigen at an end point as early as 2 hrs after placing the B cells on PLBs containing different densities of antigen (20, 100, and 200 molec/µm) (Science, 2006, 312 738-41).
Further studies will provide needed information on how the process of PLB regeneration is regulated.
Therefore, we had to design a novel bilayer system which would allow formation of PLBs on the horizontal axis and use it like a "specimen slide" to interrogate the bilayer with the FRAP system.
Protein concentration measurements were performed on corresponding PLB lysates by Quick Start™ Bradford Protein Assay Kit (Bio-Rad) and analyzed by interpolating to standard curve according to the manufacturer's instruction.
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