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RevC3, revC3 C/A and parental cell xenografts were grown by s.c injection of 1 × 10 cells (0.1 ml of serum-free medium) into the back mid-dorsal region of 6 8-week-old SCID/ bg mice, either a single or dual flank (different cell lines injected on opposite flanks) model (Paterson Institute for Cancer Research).
Marginal and submarginal meristems on opposite flanks of the primordium initiate leaf-blade formation.
Wild-type and resistant cells were inoculated in Matrigel on opposite flanks, allowing direct comparison of the treatment effects on the two xenografts whilst reducing the number of animals required for each study.
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In an independent experiment, experimental groups of six mice were injected with cells on one flank only and treated with buffer or antibody to exclude influence of the treatment on the cells on the opposite flank.
In a system that gives him licence to roam, the winger has, together with Yannick Bolasie on the opposite flank, blossomed again into one of the most dangerous wide players in the league.
24 hr later mice were s.c. injected on one flank with QDova (containing 50 µg ovalbumin), and on the opposite flank with QD included in 50 µl complete Freund's adjuvant.
Jermaine Pennant, on the opposite flank to Etherington, was the one initially bright light, dimmed by a series of heavy tackles.
Finally, we assessed whether combination treatment with intermittent chemotherapy and anti-CD137 mAb exerted an antitumor effect on tumors not treated with mAb on the opposite flank of the mice.
To determine whether a higher inoculum would unveil a PVL virulence role, CD1 mice were inoculated subcutaneously on one flank with 109 CFU of a PVL+ strain, and on the opposite flank with an equal inoculum of an isogenic PVL− strain.
Routledge, whose spectacular volley against Albion was probably the best goal anywhere on the day, is a complementary threat on the opposite flank and it is not just at the Liberty that they are convinced these two represent a more penetrative alternative to James Milner and Andros Townsend, who are preferred by England.
Sinfield's vision was the catalyst, finding James Duckworth on the right after Hall kept the ball alive on the opposite flank, and the young winger moved the ball inside to Watkins for his second score.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com