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Hybridizations were performed using the Agilent Gene Expression Hybridization Kit and each biological replicate was individually hybridized on one array.
Six samples were run on one array and the samples from the groups were randomly allocated.
For example, 20 pooled T. alpinus libraries were hybridized on one array.
It was fabricated using spotted oligo technology, which limits the number of probes that can be included on one array.
We have performed replicates on one array of the "Mouse ChIP-chip 2.1M Whole-Genome Tiling sets", which covers 10% of the genome.
On one array pattern, 10 different liver total RNA samples from 5 controls and 5 drug treated rats were spotted, each in 10 replicates, making an array of 100 spots on an area of 18 × 18 mm.
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You just stack one one array on top of the other and boom, double the size.
Specifically, researchers studying gene expression in brain tend to analyze more than one brain region on more than one array.
Of the 31,042 probe sets (genes) present on the array, 27,061 were detected significantly above background on at least one array set.
For 8 of the contigs higher expressed in the toxic strains, the signals of the non-toxic strain were identified as not "well above background" by the microarray data extraction software on at least 8 of the 12 arrays, and on at least one array for each strain-time-point combination.
d Reported to be a constituent of the H. pylori core genome, based on at least one array analysis [ 18- 20].
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