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Furthermore, our WPF values are based on microscopic counting of intact spores.
In these cases, current clinical classification depends on microscopic counting of these cells, 13, 14 although nuclear indentation in the abnormal lymphocytes is often ambiguous.
In addition, in DBA patients, the TUNEL method was performed according to the manufacturer's protocol (kit ApopDETEK, DAKOCytomation, Enzo Diagnostics Inc., Farmingdale, NY, USA) on the cells isolated from erythroid colonies and the percentage of apoptotic cells was determined based on microscopic counting of 200 cells.
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Spore suspension was adjusted to a final concentration of 1 × 10 conidia/ml based on microscopic counts using a Bürker chamber.
The number of colonies was assessed by microscopic counting.
Unlike direct microscopic counts where all cells, dead and living, are counted, CFU measures viable cells.
Reticulocyte count was made by microscopic counting on blood smears after vital staining with new methylene blue (reticulocyte stain; Sigma, St . Louis MO, USA).
Red blood cell (RBC), hematocrit, and hemoglobin (Hb) concentration were assessed in whole blood EDTA by using an automatic Coulter Counter (Beckman Coulter Inc., CA, USA); reticulocyte (RET) count was performed by microscopic counting on blood smears after vital staining with New Methylene Blue (Reticulocyte Stain, Sigma-Aldrich, St . Louis MO, USA).
Estimates of total cell mass based on direct microscopic counts and DCW determinations revealed that the average mass of a cell in − N was 81% of that in + N, confirming that the change in TAG was independent of changes in DCW.
On the next day, the adherent cells were evaluated by direct microscopic counting of four randomly chosen visual fields.
Parasitemia was determined by microscopic counting of blood smears or by fluorescence-assisted cell sorting [FACS].
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