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The initial number of colony forming units (cfu) for each experiment was determined by spreading samples from serial dilutions on KMB.
Additional strains from environmental samples were isolated by plating serial dilutions on KMB supplemented with antibiotics at the following concentrations: chloramphenicol 13 μg ml−1, ampicillin 40 μg ml−1 and cycloheximide 100 μg ml−1.
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Each larva was then homogenized in 50 µl of sterile distilled water, serially diluted, and the dilutions were spread on KMB.
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Strains were then inoculated into culture tubes containing 5 ml of KMB broth and incubated with shaking (200 rpm) overnight prior to harvest by centrifugation.
The research leading to these results has received support from the Innovative Medicines Initiative Joint Undertaking (EU FP7/2007-2013) under grant agreement no 115151 and from Cancer Research UK (Grant 17242 to KMB and the CRUK-EPSRC Imaging Centre in Cambridge and Manchester (KMB PI, KJW Co-I; reference 16465) and grant A11562 to JRG).
The research was funded by the National Science Foundation (MCB 07-44999) to LCS and a Weintraub research fellowship to KMB.
Inoculum for larval feeding experiments was obtained by culturing strains of P. fluorescens on King's Medium B (KMB) [51] and incubating plates overnight at 27°C.
Bacteria used in this study were cultured on King's medium B (KMB) agar plates, or in lysogeny broth (LB) at 27 °C [ 43– 43].
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