RB had the least effect on isolate #46.
Of course, factors other than DNA extraction efficiency may contribute to differences between the predicted number of reads based on isolate DNA extraction and the observed number of reads.
Halobacterium sp. NRC-1, Saccharomyces cerevisiae S288C, and Lactococcus lactis cremoris SK11 were under-represented in all libraries relative to the prediction based on isolate DNA extraction, whereas Acidothermus cellulolyticus and Shewanella amazonensis SB2B were over-represented in every library.
Based on isolate growth on nutrient-rich media, these strains could be grouped into 24 distinct morphotypes, 14 of which were obtained from individuals belonging to both wasp species, while five were unique to each species.
Collectively, our phylogenetic and molecular clock analyses, as well as information on isolate frequencies and global geographic distribution, facilitate the most comprehensive description to date of the global diversity and historical transmission patterns of this pathogen.
The separation of the phylogeny of CC199 based predominantly on isolate tissue source (i.e. carriage vs. disease), combined with the similar fitness of isolates by serotype, indicates that genetic factors other than serotype impact on fitness for tissue-specific virulence.
For example, because they have been shown to be more recalcitrant to lysis, one might expect that the organisms with the Gram-positive cell wall structure might consistently be under-represented in our libraries relative to the prediction based on isolate DNA extraction.
Of course, this prediction based on isolate DNA extraction (DNA quantification) does not provide a perfect expectation of the relative organism abundance in extractions of mixed communities, but it does, at least theoretically, better account for the effects of DNA extraction efficiency and genome copy number per cell.
Table 2 shows comparisons by county on isolate characteristics.
The in vitro activities of enrofloxacin on isolate AH10 were subsequently investigated.
However, depending on the study design, the expression may be based on isolate, patient, episode and/or resistance phenotype [ 46].
