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Clustering based on genes also showed that genes were expressed similarly based on functional category.
Genes were classified based on functional category and pathways using Gene Spring GX Software and Genotypic Biointerpreter-Biological AnalySoftware and Genotypic Biointerpreter-Biological
Analysis of genes based on functional category was performed to measure the differences and the similarities between the two adipocyte populations.
The selection of these OCs was initially based on functional category variation among the ORFs and OCs with ORFs with long IRs.
Differentially regulated genes were clustered using hierarchical clustering based on Pearson's coefficient correlation algorithm to identify significant gene expression patterns genes were classified based on functional category and pathways using GeneSpringGX Software and Genotypic Biointerpreter -Biological Analysis Software.
In order to test the ability of our functional network to accurately infer reliable functional annotations and the stage of symbiosis where components of the Symbiosis Interactome may participate, we investigated a basic network-based approach based on functional category membership within predicted functional modules.
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Of the total 47 genes from both SNP and methylation PSFs, we identified 13 shared functional clusters based on functional categories, gene ontology (GO) and pathway information.
Information on functional categories was extracted from the IRIS database (Kelley et al. 2005).
We further investigated their relationship by decomposing it based on functional categories, and identified categories with significant tendencies.
S. hexandrum transcript sequences were searched against MIPS Functional Catalogue (FunCat) [ 29] database to classify transcripts based on functional categories classified at MIPS.
Statistical analysis was performed on functional categories over-represented in the groups relative to the functional representation within the human proteome (Table 1, ref. [ 23]).
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