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It is therefore very unlikely that the inhibitory effect of the MAK-5 extract on foci formation is the result of direct cell killing of the transformed cells, but instead it may exert its effect on the process of transformation by the H- ras oncogene.
This result suggests that the inhibitory effect of MAK-5 extract on foci formation is not a result of direct cell killing of the transformed cells, instead, it may exert its effect at an early stage of ras-induced transformed foci.
Immunofluorescent staining of HeLa cells with antibodies that specifically recognize each one of the three HP1 isoforms indicates that they are all nuclear proteins that are bound to chromatin and accumulate on foci in interphase (Figure S1, top).
The underlying mechanism by which both compounds act on foci requires further investigation.
Various combination of the expression vectors were cotransfected into CNE1 cells, and then the effects on foci formation were analyzed.
For example, data on foci or nodules have been used to estimate rates of initiation and proliferation, under the assumption that they are preneoplastic lesions.
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