Sentence examples for on fixed tissue from inspiring English sources

Fluorescent colocalization of vimentin (VIM) and GATA binding protein 1 (GATA1) or connexin 43 (CX43) and espin (ESPN) were carried out on fixed tissue sections using standard protocols [34], [35].

Cy2, Cy3- and Cy5-coupled secondary antibodies were from Jackson's Laboratories Orientation of SOP division was either measured at telophase in living pupae using GFP-PonLD or on fixed tissue using Pins as a polarity marker.

All turnover and stability analyses were carried out by scrolling across single z-stacks of raw images using a plug-in specifically developed for OsiriX software (http://www.osirix-viewer.com) except for density analysis on fixed tissue, which was performed on the LSM image viewer software (Carl-Zeiss, Göttingen, Germany).

Since, as mentioned above, the ET-labeling intensity produced by applying the toxin on acute (i.e. still living) slices was higher than that observed when applying ET on fixed tissue, we analyzed double (ET, cell marker) staining in slices submitted to ET 10−7 M for 5 min before washing out and fixation (see the Material and Methods).

Anti-CD123 staining is not as good on fixed tissue.

Sometimes the immunohistochemical technique was performed without prior reaction for unmasked epitope on fixed tissue.

Further, the advocated approach does not require any pre-labeling, and can be carried out on fixed tissues.

Since these stainings were done on fixed tissues, they reflect mitochondrial abundance, but not mitochondrial activity.

The following antibodies were tested on fixed tissues adjacent to samples subjected to microarray.

This antigen is expressed exclusively on fixed tissue-resident macrophages [ 48, 49].

Initially, immunohistochemical detection was performed on frozen tissues as the available antibodies had lower affinity on fixed tissues.