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Human 3028: A/Vietnam/3028/2004 Avian 933: Aviancken/Vietnam/949B2004 AvInn 949B: A/conclusionetnam/949B/2004 In conclusion, we have reported an efficient, specific and sensitive assay that has been evaluated on field specimens to be able to detect a wide variety of H5N1 influenza virus isolates.
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Validation on 145 field specimens from Vietnam and Malaysia showed that the assay was specific without cross reactivity to a number of other infuenza strains as well as human respiratory related pathogens.
By using standard PCR on 16S rDNA, ftsZ, wsp and all MLST loci, Wolbachia was not detected in any DNA extract of 45 field specimens collected from Australia, Chile, Japan, South Africa, Spain and United Kingdom.
Field emission property was tested and the turn-on field of 2.4 V/μm was obtained for the specimen containing 2.7 wt.% Ni nanopowder.
The electrodeposition of zinc on field ion microscope tip specimens was attempted.
This method employed a single method of collection and relied on field morphospecies when sorting specimens for analysis.
This method was based on the time targets associated with collection, the use of a single methodology during collection, and a reliance on field morphospecies when sorting specimens for analysis.
Infact, although the analysis of a small number of field samples has been reported, this is a first step in the field validation of our RRT-PCR assay and more extensive work, involving the application of the assay on field samples, including also specimens from poultry, will be necessary in the future.
However, as pointed out by Nekaris and colleagues, this assessment was based on field research data and historic museum specimens, and cannot be considered reliable, as "loris 'presence' is usually not determined first-hand, and it also cannot be presumed that lorises still occur in areas from where they were once collected".
Our data showed that genetic markers can allow the revision of taxonomic identifications based on field observations and inspection of herbarium specimens.
The two often rode horseback on field expeditions, engaged in intense discussions of evolution, intermittently stopping to collect specimens.
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