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Because of the antiparallel nature of the DNA strands, new strand synthesis is different on each template.
Beginning at the origin of replication, a complex enzyme called DNA polymerase moves along the DNA molecule, pairing nucleotides on each template strand with free complementary nucleotides.
Models based on each template were generated using NEST software [29].
Models based on each template were constructed using the program NEST [29].
Sequencing reactions were performed on each template on the automated ABI PRISM 377 DNA Sequencer (MACROGEN Inc., Korea).
MDA occurs through a process where random hexamers prime multiple times on each template strand (Fig 2A).
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On top of each template, primer extension direction is indicated by → whereas × marks a non-extended scenario.
Sample cycle threshold (Ct) values were standardized for each template based on a GAPC control primer reaction, and the comparative Ct method [ 37] was used to determine the relative transcript abundance of each gene.
Sample cycle threshold (ct) values were standardized for each template based on reference gene control primer reaction, and the 2 ∆∆CT method was used to analyze relative changes in gene expression [ 28].
On average, each template should take only 1 or 2 min to examine and grade.
Lay each template on the dough and cut out the house pieces.
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