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Especially for mitochondrially localized pathways such as citric acid cycle and oxidative phosphorylation the pulses are very broad, with a neat downregulation only in correspondence of the bursts of transcription and an overall profile often exhibiting a double peak on each period (occurring with a phase lag of ~100° one from the other, see Fig. S8 in Additional file 1).
Univariate and multivariate analyses were performed on each period separately.
We observed 100 bedside interactions on each period.
In addition, treatment comparisons were performed focusing on each period separately (between patient comparisons).
For each gene, the pulse width is computed estimating on each period the interval in which the expression level stays above the median value across consecutive samples.
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Plasma hormone and metabolite concentrations in blood samples taken from the jugular vein were measured on the morning of feeding on day 14 of each period.
Fecal pH was measured on day 7 of each period.
All cows received an intravenous glucose tolerance test (GTT) on d 5 of each period.
On d 21 of each period, liver tissue was collected by percutaneous liver biopsy.
Liver and muscle were biopsied on d 14 of each period.
On day 14 of each period of the digestibility experiment, total urine and feces outputs were collected and weighed.
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