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Three replicates were done on each concentration of sago hampas.
Three replicates of qPCR were performed on each concentration using the SWS2A assay.
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The number of coughs was counted during a 2 min inhalation of each capsaicin solution and for additional 1 min. The total number of coughs during the 3 – min period was recorded on the inhalation of each concentration of capsaicin.
In Ferric ion reducing power assay, MEFCA showed strong reducing power forming blue or green coloured complex with reduction of Fe3+/ferricyanide complex in to the ferrous form depending on the reducing power of each concentration of the extract.
The effects of JMV 2959, a GHS-R1A antagonist, on preference for each concentration of ethanol and sucrose were then tested.
Additionally, electrophysiologically determined inhibitory doses (IC50/IC80) were used to compare the effects on cell viability (for each concentration n≥4 well).
Cells were incubated with the samples (three wells on a plate for each concentration) for 24 to 72 h.
Cells were incubated with the samples (three wells on a plate for each concentration) from 48 to 72 h.
Larvae were treated with prazosin over range of concentrations (n = 12 for each concentration) on the morning of day 5 of development.
For the beating rate, the change was evaluated by the ANOVA for the multiple regression of ln % beating rate on ln time for each concentration of doxorubicin alone, with monoHER or with AdCat preinfection.
Five neonates (younger than 24 h old) were used for each concentration based on 4 replications.
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