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Next, we identified robust Wnt target genes based on consistent changes in a combination of experiments (details not shown): lithium chloride inhibition of GSK3β 21 in the normal RIE-1 intestinal cell line (Leedham, unpublished data, 2010) and exon mRNA expression microarray and qRT-PCR in tumours from Apc-mutant mice.
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The interventions focus on either small, consistent changes in eating and exercise behaviors, or larger, periodic changes to buffer against expected weight gains.
Based on this result, we focused on probe sets showing consistent changes of expression in the first 12 hours of the time course in at least two of the three cell lines.
Furthermore, analyses of the mood and arousal measures detected consistent changes on arousal subscales and caffeine administration elevated saliva cortisol.
These data documented consistent changes on the functional limitation and disability levels up to 6 months posttreatment, but not on the societal outcome level.
Vancomycin has been reported to induce consistent changes on intestinal microbial structures of humans treated with that drug [ 32], but another study reported unpredictable changes caused by the same drug [ 33].
Metal ion binding was found to be among the top 9 GO terms significantly regulated in the cross-study analysis of psychosis (p = 0.01) and was chosen to highlight based on the robust and consistent changes in a shared gene group (i.e. metallothionein genes).
Therefore, we focused on the genes that showed consistent changes in response to food deprivation in both sets of mice (comparisons 3 and 4 combined), since there are hardly any differences between the data for the two genotypes (see below).
A subset of 43 paired extracts analysed on Western blots also revealed consistent changes in cathepsin H protein forms in cancers.
However, we did find differential expression of a number of genes between treated and control mice, and, based on these semiquantitative results, real-time reverse transcription PCR was performed on selected genes that exhibited consistent changes in expression by spironolactone by microarray analysis and were of potential pathogenic interest.
Next, we focused on the 38 probes that showed consistent changes with age in all regions (Fig. 2A).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com