Sentence examples for on column with from inspiring English sources
Column chromatography separations were carried out on column with Merck Kieselgel 60 or Aluminium oxide 90, neutral (70 230 mesh).
Yield of activity retained on column with 111In-HEPES (1M) varied 31 98% and was pH and volume dependent.
DNA digestion was performed on column with RNase-free DNase.
RNA were extracted with Plant RNeasy (Qiagen, The Nederlands) and treated on column with Dnase (Qiagen).
The standard curves were linear in a range between 0.0 and 100 pmol on column with correlation coefficients (R)>0.98.
Total RNA of 5 10 eggs or larvae was extracted using TRIzol (Invitrogen) after which the RNA was purified and DNA digested on column with the RNeasy kit (Qiagen).
RNA was treated on-column with RNase-free DNase (QIAGEN) to remove contaminating genomic DNA.
We labeled DNA "on-column" with SYBR gold/Tris Gly separation buffer (1 100,000).
Leftovers of DNA were digested on-column with RNAse-free DNase I Set (Quiagen) for 15 minutes.
NEDD4, NEDD4L, Rsp5WW3-HECT, and Rsp5HECT proteins used in the assays were pulled down by affinity chromatography and cleaved from their tags on-column with TEV.
Total RNA was isolated from PBMCs using RNeasy Mini Kit (Qiagen) and DNase digestion was performed on-column with the RNase-Free DNase Set (Qiagen) according to manufacturer's protocol.
