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Ongoing studies will determine whether iAs inhibits HAT activity of CBP and post-translational modifications on CBP or p300 that can influence HAT activity to begin to determine their role in transcriptional repression by iAs.
We tested effects of DDT on CBP activity using a mammalian one-hybrid assay in which the full-length CBP is tethered to GAL4-DBD in conjunction with a GAL4 responsive luciferase reporter.
Future efforts will require efficient xylose utilization, GX cleavage by a β-glucosidase, and/or a CBP with improved substrate specificity to overcome the negative impacts of xylose on CBP in cellobiose and xylose co-fermentation.
We therefore tested the effect of xylose on CBP cellobiose fermentation, as well as two potential approaches to alleviate inefficient CBP-mediated cellobiose fermentation in the presence of xylose.
Even if parents are not informed of the type of practice model before registering their children with a CBP, we cannot exclude that family characteristics, such as anxiety, could have an effect either on CBP selection or on use the ED.
To investigate whether the HAT activity of CBP is directly involved in the regulation of insulator activity at these sites, we treated S2 cells with the CBP inhibitor C646 [ 35] or with C37 (a compound similar to C646 that shows no effect on CBP HAT activity, [ 35]) and used H3K27me3 antibodies in ChIP-qPCR.
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Thirteen consensus BP QTL were identified using a meta-analysis approach [ 30], two of which are on LG5 and one, cBP-12 QTL, is located on HICF contig 23 [ 24].
Xylose exerted negative impacts on CBP-mediated cellobiose fermentation by acting as a substrate for GX byproduct formation and a mixed-inhibitor for cellobiose phosphorylase activity.
Current studies on CBP-based hydrogen production mainly focus on using the thermophilic cellulolytic bacterium Clostridium thermocellum and the extremely thermophilic cellulolytic bacterium Caldicellulosiruptor saccharolyticus.
Moreover, current studies on CBP-based hydrogen production mainly concentrate on using co-cultures of the thermophilic cellulolytic bacterium Clostridium thermocellum with non-cellulolytic thermophilic anaerobic bacteria and the extremely thermophilic cellulolytic bacterium Caldicellulosiruptor saccharolyticus[ 12, 13].
In order to investigate the effect of the P65A mutation on CBP-dependent transcription, we monitored the expression of CBP target genes — defined as being responsive to CBP — upon overexpression of wild-type TDG in comparison to the overexpression of TDG P65A.
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