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"At a minimal cost to the government, this amendment will have an immediate, positive and direct impact on Canada's ability to grow a robust Canadian technology industry," explains Terry Matthews, Chairman , Wesley Clover
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2,4,6-Trinitrobenzenesulfonic acid (TNBS) was purchased from Sigma-Aldrich Canada Ltd. (Oakville, ON, Canada).
All chemicals for media and substrates were obtained from Sigma-Aldrich Canada Co. (Oakville, ON, Canada).
This analysis was carried out using Simple PCI software (Nikon Canada Inc., Mississauga, ON, Canada).
The colour was developed with DAB (DAKO Diagnostics Canada Inc., Mississauga, ON, Canada) without nickel chloride.
One microgram of total RNA was reverse-transcribed using Moloney murine leukaemia virus reverse transcriptase (Fermentas Canada Inc., Burlington, ON, Canada) as detailed in the manufacturer's guidelines.
The research ethics board of St. Joseph's Hospital (Hamilton, ON, Canada) approved this protocol.
The procedure was performed according to the manufacturer's recommendations (Qiagen, Mississauga, ON, Canada).
Sucralose was purchased from Sigma Aldrich (Oakville, ON, Canada).
Oseltamivir phosphate (OP) free base (Hoffmann-La RONhe Ltd, Mississauga, ON, Canada) was used at the concentrations indicated.
Amplified IP or TI DNA samples (2 µg) was labelled with Cy5-dUTP or Cy3-dUTP (Perkin Elmer Life Sciences, Woodbridge, ON, Canada), respectively, using CGH kits (Invitrogen, Burlington, ON, Canada).
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