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Therefore, site-saturation mutagenesis was performed on both positions and a complete set of all 38 variants was subjected to rapid HPLC screening for improved ampicillin synthesis.
Amino acid conservation was strong on both positions (86% for βThr656Ile and 100% for γPro674Ser, Additional file 1: Table S1).
It appears here that slight differences in SAM and/or substrate binding in the active site lead to the enhanced functionality observed with Cfr to act on both positions C2 and C8 of A2508 as compared to the singly active RlmN at site C2.
When using the "pairs trade" hedging strategy, you can include stop-loss orders on both positions.
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In other words, the oddsmaker is stingy: it won't give us good odds on both position and velocity at once.
This corresponds to the unavailability of any a priori information on both position and Lagrange multipliers and is therefore a worst case scenario.
Given that states are clustered on both position and velocity, the transition matrix is an implicit constraint upon the second derivative (acceleration) of speech lip movements.
In this paper, the most commonly used shear strength correlations were ranked based on both position conformity and trend conformity using amended Theil inequality coefficient (ATIC) method as proposed by Song et al. [28].
Furthermore, the agent's model allows for random fluctuations on both position and velocity.
From this result, we find the protein has two obvious changes on both position and even composition; then we suggest the structure might make Ras lose the function and be inhibited.
Three of them, T4SEpre_Joint trained on joint features of position-specific Aac, Sse and Acc, T4SEpre_bpbAac trained on Bi-Profile Bayesian Aac, and T4SEpre_psAac trained on both position-specific (Single-Profile Bayesian) and sequence-based Aac features, considerably outperformed the others in terms of sensitivity, specificity, accuracy, AUC and MCC (Table 1 and Figure 4).
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